A description with anatomical pictures of a simple and gentle dorsolateral approach to the rabbit sciatic nerve are presented. Blunt separation of the septum between the semitendinosus muscle and the caput pelvinum bicipitis femoris muscle, allows for excellent access to the nerve along a considerable length. The dorsolateral subfascial vein of the thigh is an easily identifiable landmark of this septum. This gentle approach ensures very smooth healing.