Abstract
Novel N-substituted phthalimides (2-substituted 1H-isoindole-1,3-diones) were prepared, and their effects on tumor necrosis factor-alpha (TNF-alpha) production by human leukemia cell line HL-60 stimulated with 12-O-tetradecanoylphorbol 13-acetate (TPA) or okadaic acid (OA) were examined. A structure-activity relationship study of the N-phenylphthalimides and N-benzylphthalimides revealed that their enhancing effect on TPA-induced TNF-alpha production by HL-60 cells and their inhibiting effect on OA-induced TNF-alpha production by HL-60 cells are only partially correlated.
MeSH terms
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Cell Survival / drug effects
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Dose-Response Relationship, Drug
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HL-60 Cells / drug effects*
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HL-60 Cells / physiology
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Humans
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Immunologic Factors / chemical synthesis
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Immunologic Factors / chemistry*
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Immunologic Factors / pharmacology*
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Structure
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Okadaic Acid / pharmacology
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Optical Rotation
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Phthalimides / chemical synthesis
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Phthalimides / chemistry*
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Phthalimides / pharmacology*
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Structure-Activity Relationship
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / biosynthesis*
Substances
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Immunologic Factors
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Phthalimides
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Tumor Necrosis Factor-alpha
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Okadaic Acid
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Tetradecanoylphorbol Acetate