This study investigates the vascularization of the Achilles tendon in 32 lower limbs of human neonates by injecting colored latex with subsequent microdissection. Because of its biomechanical significance (achilleo-calcaneal-plantar system) and the lack of descriptions in the literature, we analyzed the vascularization of the attachment area in the calcaneus, describing the achilleo-calcaneal vascular network, which is always well supplied in contrast to other tendinous areas.