Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases

J Damborsky; M G Nyandoroh; M Nĕmec; I Holoubek; A T Bull; D J Hardman

Some biochemical properties and the classification of a range of bacterial haloalkane dehalogenases

Biotechnol Appl Biochem. 1997 Aug;26(1):19-25.

Authors

J Damborsky¹, M G Nyandoroh, M Nĕmec, I Holoubek, A T Bull, D J Hardman

Affiliation

¹ Laboratory of Environmental Microbiology, Masaryk University, Brno, Czech Republic.

PMID: 9261999

Abstract

Multivariate analyses and experimental data have been used to evaluate the relationships between eight bacterial hydrolytic haloalkane dehalogenases. The results indicate that seven of the dehalogenases investigated can confidently be placed into two Classes [sensu Slater, Bull and Hardman (1995) Biodegradation 6, 181-189] according to their substrate profiles. The remaining enzyme, isolated from Rhodococcus erythropolis CP9, appears to represent a third Class of haloalkane dehalogenases.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteria / enzymology*
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Hydrolases / chemistry
Hydrolases / classification
Hydrolases / metabolism*
Molecular Sequence Data
Sequence Homology, Amino Acid

Substances

Hydrolases
haloalkane dehalogenase