CD62, thromboxane B2, and beta-thromboglobulin: a comparison between different markers of platelet activation after contact with biomaterials

J Biomed Mater Res. 1997 Sep 5;36(3):289-94. doi: 10.1002/(sici)1097-4636(19970905)36:3<289::aid-jbm3>3.0.co;2-a.

Abstract

The authors examined the modifications of some markers of platelet activation after contact with biomaterials. Glycoprotein GMP-140 (CD62) was evaluated by flow cytometry; beta-thromboglobulin (beta-TG) and thromboxane B2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a significant increase in beta-TG and TXB2, with no increase in CD62 on the nonadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of beta-TG and TXB2 were noted. The increase was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials*
  • Biomarkers
  • Cell Adhesion
  • Humans
  • P-Selectin*
  • Platelet Activation* / drug effects
  • Thromboxane B2*
  • beta-Thromboglobulin*

Substances

  • Biocompatible Materials
  • Biomarkers
  • P-Selectin
  • beta-Thromboglobulin
  • Thromboxane B2