Cytogenetic analysis of Liza ramada (Pisces, Perciformes) by different staining techniques and fluorescent in situ hybridization

Heredity (Edinb). 1997 Jul:79 ( Pt 1):83-7. doi: 10.1038/hdy.1997.125.

Abstract

A cytogenetic investigation was carried out on specimens of Liza ramada, a mugilid species common in the Mediterranean sea. The analysis of chromosomes was performed through Ag-staining, C-banding, chromomycin A3 and DAPI staining, and fluorescent in situ hybridization with ribosomal genes. The results obtained are discussed with respect to cytotaxonomic implications and to the features of NORs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosome Banding
  • Cytogenetics
  • DNA Probes
  • DNA, Ribosomal / genetics
  • Fluorescent Dyes
  • Humans
  • In Situ Hybridization, Fluorescence
  • Indoles
  • Karyotyping
  • Nucleolus Organizer Region / ultrastructure
  • Perciformes / genetics*
  • RNA, Ribosomal, 18S / genetics
  • Silver
  • Staining and Labeling / methods

Substances

  • DNA Probes
  • DNA, Ribosomal
  • Fluorescent Dyes
  • Indoles
  • RNA, Ribosomal, 18S
  • Silver
  • DAPI