Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

Appl Environ Microbiol. 1997 Aug;63(8):3336-40. doi: 10.1128/aem.63.8.3336-3340.1997.

Abstract

Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / genetics*
  • Cloning, Molecular
  • DNA Probes / genetics
  • Endo-1,3(4)-beta-Glucanase*
  • Gene Expression
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / metabolism*
  • Lactobacillus / genetics*
  • Lactobacillus / metabolism*
  • Plasmids
  • Promoter Regions, Genetic / genetics
  • Recombination, Genetic
  • Restriction Mapping
  • beta-Galactosidase / metabolism

Substances

  • DNA Probes
  • Glycoside Hydrolases
  • beta-Galactosidase
  • Endo-1,3(4)-beta-Glucanase