Formation of homo- and heterooligomeric supramolecular structures by D-glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase in reversed micelles of aerosol OT in octane

Biochem Mol Biol Int. 1997 Jul;42(3):527-34. doi: 10.1080/15216549700202931.

Abstract

The supramolecular structure of oligomeric enzymes can be specifically regulated by changing the size of an inner cavity of Aerosol OT reversed micelles in octane. Both D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and lactate dehydrogenase (LDH) reveal an ability to exist and function in monomeric, dimeric and tetrameric forms (homooligomers). Various heterooligomeric complexes, in particular, GAPDH monomer--LDH monomer, GAPDH dimer--LDH tetramer were detected in reversed micelles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catalysis
  • Dioctyl Sulfosuccinic Acid
  • Glyceraldehyde-3-Phosphate Dehydrogenases / chemistry*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • L-Lactate Dehydrogenase / chemistry*
  • L-Lactate Dehydrogenase / metabolism
  • Macromolecular Substances
  • Micelles*
  • Octanes
  • Peptide Fragments / chemistry*
  • Peptide Fragments / metabolism
  • Polymers
  • Protein Conformation
  • Rabbits
  • Solvents
  • Surface-Active Agents

Substances

  • Macromolecular Substances
  • Micelles
  • Octanes
  • Peptide Fragments
  • Polymers
  • Solvents
  • Surface-Active Agents
  • Dioctyl Sulfosuccinic Acid
  • glyceraldehyde 3-phosphate dehydrogenase (304-313)
  • L-Lactate Dehydrogenase
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • octane