Abstract
40 and 60 S ribosomal subunits have been reconstituted in vitro from purified ribosomal RNA and ribosomal proteins of Dictyostelium discoideum. The functionality of the reconstituted ribosomes was demonstrated in in vitro mRNA-directed protein synthesis. The reassembly proceeded well with immature precursors of ribosomal RNA but poorly if at all with mature cytoplasmic RNA species. Reassembly also required a preparation of small nuclear RNA(s), acting as morphopoietic factor(s).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Dictyostelium
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RNA Precursors / metabolism
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RNA, Fungal / metabolism*
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RNA, Messenger / metabolism
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RNA, Protozoan / metabolism*
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RNA, Ribosomal / metabolism
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RNA, Ribosomal, 5.8S / metabolism
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RNA, Small Nuclear / metabolism
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Ribosomal Proteins / metabolism*
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Ribosomes / metabolism*
Substances
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RNA Precursors
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RNA, Fungal
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RNA, Messenger
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RNA, Protozoan
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RNA, Ribosomal
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RNA, Ribosomal, 5.8S
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RNA, Small Nuclear
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Ribosomal Proteins