Reconstitution of functional eukaryotic ribosomes from Dictyostelium discoideum ribosomal proteins and RNA

J Biol Chem. 1997 Aug 8;272(32):19682-7. doi: 10.1074/jbc.272.32.19682.

Abstract

40 and 60 S ribosomal subunits have been reconstituted in vitro from purified ribosomal RNA and ribosomal proteins of Dictyostelium discoideum. The functionality of the reconstituted ribosomes was demonstrated in in vitro mRNA-directed protein synthesis. The reassembly proceeded well with immature precursors of ribosomal RNA but poorly if at all with mature cytoplasmic RNA species. Reassembly also required a preparation of small nuclear RNA(s), acting as morphopoietic factor(s).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Dictyostelium
  • RNA Precursors / metabolism
  • RNA, Fungal / metabolism*
  • RNA, Messenger / metabolism
  • RNA, Protozoan / metabolism*
  • RNA, Ribosomal / metabolism
  • RNA, Ribosomal, 5.8S / metabolism
  • RNA, Small Nuclear / metabolism
  • Ribosomal Proteins / metabolism*
  • Ribosomes / metabolism*

Substances

  • RNA Precursors
  • RNA, Fungal
  • RNA, Messenger
  • RNA, Protozoan
  • RNA, Ribosomal
  • RNA, Ribosomal, 5.8S
  • RNA, Small Nuclear
  • Ribosomal Proteins