Effects of brefeldin A on the expression and transport of influenza A virus haemagglutinin, M1 and M2 proteins within the cell

Acta Virol. 1997 Apr;41(2):83-91.

Abstract

Brefeldin A (BFA) decreased the expression of influenza A virus haemagglutinin (HA) and M2 protein on the plasma membrane of virus-infected MDCK cells. It caused a retention of M1 protein in the cell nucleus and a decrease of its expression on the plasma membrane. On the other hand, an increased labelling of the cytoplasmic domain of M2 protein on the plasma membrane in BFA-treated cells was observed in contrast to the labelling in BFA-untreated cells. The effects of BFA on the microtubules and cellular motors are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • Biological Transport / drug effects
  • Brefeldin A
  • Cell Compartmentation / drug effects
  • Cell Line
  • Cyclopentanes / pharmacology*
  • Dogs
  • Hemagglutinins, Viral / metabolism*
  • Immunohistochemistry
  • Ion Channels / metabolism
  • Membrane Proteins / metabolism
  • Viral Matrix Proteins / metabolism*

Substances

  • Antigens, Surface
  • Cyclopentanes
  • Hemagglutinins, Viral
  • Ion Channels
  • M-protein, influenza virus
  • M1 protein, Influenza A virus
  • M2 protein, Influenza A virus
  • Membrane Proteins
  • Viral Matrix Proteins
  • Brefeldin A