The aim of this study was to compare, in vitro, the machining efficiency of different triangular cross-section K-files made of nickel titanium (Nitiflex, Naviflex), titanium (Microtitane), and stainless steel (Flexofile, Flex-R). Ten instruments of each K-file from size 25 to 40 were tested. The cutting efficiency was assessed in a linear motion using an indentation caliper to measure the depth of grooves. The load applied (in grams) was equal to the ISO file size. Each file was allowed to do 100 back-and-forward movements. Files made of stainless steel were the most effective, in particular Flexofile. There were statistically significant differences between Flexofile and Flex-R in all sizes. In the group of nickel titanium instruments, Nitiflex was significantly more efficient than Naviflex in all sizes. The machining ability of titanium files was higher than that of Naviflex but lower than that of Nitiflex and stainless steel files.