This study was performed to determine the reliability and validity of a new isokinetic squat device in comparison to knee-extension tests performed using a Cybex. Athletic male subjects (n = 29) performed a series of isokinetic squat tests at 0.4 m.s-1, knee-extension tests at 1.05, 2.09 and 3.14 rad.s-1, and a 6-s stationary cycle test which was used as the measure of functional performance. The squat tests included a purely concentric squat without pre-load, a test with pre-load and a stretch-shorten cycle test. Two trials of each test were performed on one testing occasion. Intraclass correlation co-efficients (r = 0.89-0.96) and co-efficients of variation (3.1-8.7%) were determined between trials, and these indicated that all of the tests were highly reliable. The velocity characteristics of the newly developed system demonstrated that it was an effective isokinetic device, with the mean velocity of 0.41 m.s-1 varying within narrow limits, a relatively small velocity overshoot and an isokinetic portion of movement of approximately 80%. The squat tests demonstrated a higher relationship to cycling performance (r = 0.57-0.65) as compared to the knee-extension tests (r = 0.45-0.51). This difference was amplified when a more homogeneous group of subjects was examined. Further, the squat tests were superior to the knee-extension tests in discriminating between differing levels of cycling performance ability. These differences were believed to be due to the greater specificity of the squat movement, in comparison to the knee extension, to the performance of interest.