Abstract
Tip of herpesvirus saimiri associates with Lck and down-regulates Lck-mediated activation. We identified a novel cellular Tip-associated protein (Tap) by a yeast two-hybrid screen. Tap associated with Tip following transient expression in COS-1 cells and stable expression in human Jurkat-T cells. Expression of Tip and Tap in Jurkat-T cells induced dramatic cell aggregation. Aggregation was likely caused by the up-regulated surface expression of adhesion molecules including integrin alpha, L-selectin, ICAM-3, and H-CAM. Furthermore, NF-kappaB transcriptional factor of aggregated cells had approximately 40-fold higher activity than that of parental cells. Thus, Tap is likely to be an important cellular mediator of Tip function in T cell transformation by herpesvirus saimiri.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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COS Cells
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Cell Adhesion Molecules / biosynthesis
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Cell Aggregation / genetics
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Cloning, Molecular
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DNA, Complementary / isolation & purification
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Humans
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Jurkat Cells
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Molecular Sequence Data
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NF-kappa B / metabolism
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Nucleocytoplasmic Transport Proteins*
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Phosphoproteins / chemistry
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Protein Biosynthesis
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Proteins / genetics
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Proteins / metabolism*
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RNA-Binding Proteins*
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T-Lymphocytes / metabolism
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Cell Adhesion Molecules
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DNA, Complementary
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NF-kappa B
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NXF1 protein, human
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Nucleocytoplasmic Transport Proteins
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Phosphoproteins
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Proteins
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RNA-Binding Proteins
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Viral Proteins
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tyrosine kinase interacting protein, Saimiriine herpesvirus 2