Abstract
We show that retinoid receptor antagonists applied to the presumptive wing region block the formation of a zone of polarizing activity (ZPA). This suggests a direct relationship between retinoid signaling and the establishment of the ZPA. We provide evidence that the Hox gene, Hoxb-8, is a direct target of retinoid signaling since exogenously applied RA rapidly induces this gene in the absence of protein synthesis and, moreover, retinoid receptor antagonists down-regulate Hoxb-8 expression. In addition, we find that, in the lateral plate mesoderm, the domains of Hoxb-8 expression and of polarizing activity are coextensive. Taken together, these findings support the hypothesis that retinoids are required for the establishment of a ZPA, and that retinoids act, at least in part, through Hoxb-8, a gene associated with ZPA formation (Charité et al., 1994).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Benzoates / pharmacology
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Cells, Cultured
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Chick Embryo
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Chickens
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Chromans / pharmacology
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Egg Proteins / biosynthesis*
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Embryonic Induction
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Gene Expression
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Homeodomain Proteins / biosynthesis*
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Homeodomain Proteins / chemistry
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Limb Buds / transplantation
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Membrane Glycoproteins / biosynthesis*
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Mesoderm / drug effects
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Mesoderm / physiology*
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Mice
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Molecular Sequence Data
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Receptors, Cell Surface / biosynthesis
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Receptors, Retinoic Acid / antagonists & inhibitors
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Receptors, Retinoic Acid / physiology*
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Retinoid X Receptors
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Retinoids / pharmacology
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Sequence Homology, Amino Acid
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Signal Transduction / drug effects*
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Tetrahydronaphthalenes / pharmacology
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / physiology*
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Tretinoin / pharmacology*
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Wings, Animal / embryology
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Zona Pellucida Glycoproteins
Substances
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Benzoates
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Chromans
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Egg Proteins
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Homeodomain Proteins
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Hoxb8 protein, mouse
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LG 100754
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Membrane Glycoproteins
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Receptors, Cell Surface
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Receptors, Retinoic Acid
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Retinoid X Receptors
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Retinoids
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Tetrahydronaphthalenes
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Transcription Factors
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Zona Pellucida Glycoproteins
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Ro 41-5253
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Tretinoin