Abstract
We have cloned the RAG-1 promoter region and have determined that almost all detectable promoter activity resides within a 208-bp fragment. Sequence analysis of this promoter region has identified potential recognition motifs for a number of lymphocyte-restricted and ubiquitous transcription factors. Subsequent assays have revealed that the NF-Y transcription factor interacts with a CCAAT site within the RAG-1 promoter and appears to play an important role in the positive transcriptional regulation of this gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Base Sequence
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation*
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Homeodomain Proteins*
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics*
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Proteins / genetics*
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Proteins / immunology
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Transcription Factors / genetics
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Transcription Factors / immunology
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Transcription, Genetic
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Homeodomain Proteins
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Proteins
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Transcription Factors
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RAG-1 protein