Regulation of the RAG-1 promoter by the NF-Y transcription factor

J Immunol. 1997 Jun 1;158(11):5071-4.

Abstract

We have cloned the RAG-1 promoter region and have determined that almost all detectable promoter activity resides within a 208-bp fragment. Sequence analysis of this promoter region has identified potential recognition motifs for a number of lymphocyte-restricted and ubiquitous transcription factors. Subsequent assays have revealed that the NF-Y transcription factor interacts with a CCAAT site within the RAG-1 promoter and appears to play an important role in the positive transcriptional regulation of this gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation*
  • Homeodomain Proteins*
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics*
  • Proteins / genetics*
  • Proteins / immunology
  • Transcription Factors / genetics
  • Transcription Factors / immunology
  • Transcription, Genetic

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Proteins
  • Transcription Factors
  • RAG-1 protein