Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit

Mol Biotechnol. 1997 Feb;7(1):1-4. doi: 10.1007/BF02821541.

Abstract

Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fruit / genetics*
  • RNA, Plant / isolation & purification*

Substances

  • RNA, Plant