Detection of polymorphic FGF receptor 1 mRNA in bovine lens epithelium by PCR

Biochem Mol Biol Int. 1997 May;41(6):1171-8. doi: 10.1080/15216549700202261.

Abstract

The expression of FGFR 1, 2 and 3 but not FGFR 4 was detected in bovine lens epithelial cells. FGFR 1 expression of both the 2-lg and 3-lg forms was detected by a difference in the lengths of the PCR products. Furthermore, mRNA which included or excluded six nucleotides corresponding to two amino acids in the acid box region of FGFR 1 was detected by a difference in mobility of the homo- and hetero-duplex strands formed in the PCR reaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • Clone Cells
  • DNA Primers
  • Electrophoresis
  • Epithelial Cells
  • Epithelium / metabolism
  • Fibroblast Growth Factors / metabolism*
  • Genetic Vectors
  • Lens Capsule, Crystalline / chemistry*
  • Lens Capsule, Crystalline / cytology
  • Molecular Sequence Data
  • Nucleic Acid Heteroduplexes
  • Polymerase Chain Reaction
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics*
  • Receptor Protein-Tyrosine Kinases*
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptors, Fibroblast Growth Factor / genetics*
  • Receptors, Fibroblast Growth Factor / metabolism
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • Nucleic Acid Heteroduplexes
  • RNA, Messenger
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factors
  • Receptor Protein-Tyrosine Kinases
  • Receptor, Fibroblast Growth Factor, Type 1

Associated data

  • GENBANK/D89807
  • GENBANK/D89809
  • GENBANK/M58051
  • GENBANK/M91599
  • GENBANK/X51803
  • GENBANK/X57205
  • GENBANK/Z71929