Daleformis, a new phytoalexin from the roots fo Dalea filiciformis: an inhibitor of endothelin converting enzyme

J Nat Prod. 1997 Mar;60(3):306-8. doi: 10.1021/np960654e.

Abstract

As part of a search for novel inhibitors of endothelin converting enzyme (ECE), the MeOH-CH2Cl2 extract of the roots of Dalea filiciformis was shown to be active. Bioassay-guided fractionation of the extract yielded a novel phytoalexin, daleformis (1), whose structure was determined by interpretation of spectral data and X-ray analysis. Daleformis (1) inhibited ECE with an IC50 of 9 microM.

MeSH terms

  • Acetylation
  • Aspartic Acid Endopeptidases / antagonists & inhibitors*
  • Benzofurans / isolation & purification*
  • Benzofurans / pharmacology
  • Benzopyrans / isolation & purification*
  • Benzopyrans / pharmacology
  • Chromatography, Thin Layer
  • Crystallography, X-Ray
  • Endothelin-Converting Enzymes
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology
  • Metalloendopeptidases / antagonists & inhibitors*
  • Molecular Conformation
  • Plant Roots / chemistry*
  • Plants, Medicinal / chemistry*
  • Spectrophotometry, Infrared

Substances

  • Benzofurans
  • Benzopyrans
  • Enzyme Inhibitors
  • daleformis
  • Aspartic Acid Endopeptidases
  • Metalloendopeptidases
  • Endothelin-Converting Enzymes