Production of tumor necrosis factor and nitric oxide by macrophages infected with live and dead mycobacteria and their suppression by an interleukin-10-secreting recombinant

Infect Immun. 1997 May;65(5):1931-5. doi: 10.1128/iai.65.5.1931-1935.1997.

Abstract

We have analyzed mycobacterium-induced cytokine secretion in the J774A.1 macrophage-like cell line. Tumor necrosis factor alpha (TNF-alpha) was preferentially induced by live organisms, both slow and rapid growing. Expression of interleukin-10 by a recombinant strain of Mycobacterium smegmatis caused reduced production of TNF-alpha and nitric oxide during the early stages of infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cells, Cultured
  • Humans
  • Interleukin-10 / genetics*
  • Interleukin-10 / immunology*
  • Macrophages / metabolism*
  • Macrophages / microbiology*
  • Mycobacterium / genetics*
  • Mycobacterium Infections / genetics*
  • Mycobacterium Infections / immunology*
  • Mycobacterium Infections / metabolism
  • Mycobacterium bovis / growth & development
  • Mycobacterium bovis / immunology*
  • Nitric Oxide / metabolism*
  • Recombinant Proteins / immunology*
  • Tuberculosis / immunology*
  • Tuberculosis / prevention & control*
  • Tumor Necrosis Factor-alpha / metabolism*
  • Vaccines, Inactivated / immunology

Substances

  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Vaccines, Inactivated
  • Interleukin-10
  • Nitric Oxide