Abstract
We have analyzed mycobacterium-induced cytokine secretion in the J774A.1 macrophage-like cell line. Tumor necrosis factor alpha (TNF-alpha) was preferentially induced by live organisms, both slow and rapid growing. Expression of interleukin-10 by a recombinant strain of Mycobacterium smegmatis caused reduced production of TNF-alpha and nitric oxide during the early stages of infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Western
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Cells, Cultured
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Humans
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Interleukin-10 / genetics*
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Interleukin-10 / immunology*
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Macrophages / metabolism*
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Macrophages / microbiology*
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Mycobacterium / genetics*
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Mycobacterium Infections / genetics*
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Mycobacterium Infections / immunology*
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Mycobacterium Infections / metabolism
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Mycobacterium bovis / growth & development
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Mycobacterium bovis / immunology*
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Nitric Oxide / metabolism*
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Recombinant Proteins / immunology*
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Tuberculosis / immunology*
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Tuberculosis / prevention & control*
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Tumor Necrosis Factor-alpha / metabolism*
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Vaccines, Inactivated / immunology
Substances
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Vaccines, Inactivated
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Interleukin-10
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Nitric Oxide