Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit

N P Arbatsky; A S Shashkov; G Widmalm; Y A Knirel; K Zych; Z Sidorczyk

doi:10.1016/s0008-6215(96)00308-4

Structure of the O-specific polysaccharide of Proteus penneri strain 25 containing N-(L-alanyl) and multiple O-acetyl groups in a tetrasaccharide repeating unit

Carbohydr Res. 1997 Mar 5;298(3):229-35. doi: 10.1016/s0008-6215(96)00308-4.

Authors

N P Arbatsky¹, A S Shashkov, G Widmalm, Y A Knirel, K Zych, Z Sidorczyk

Affiliation

¹ Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

PMID: 9090817
DOI: 10.1016/s0008-6215(96)00308-4

Abstract

Based on sugar and methylation analyses, O-deacetylation, Smith degradation, and 1H and 13C NMR spectroscopy, including 2D COSY, 1H-detected 1H, 13C heteronuclear single-quantum coherence (HSQC), and 1H-detected 1H, 13C heteronuclear multiple-bond connectivity (HMBC) experiments, the following structure of the O-specific polysaccharide of Proteus penneri strain 25 was established: [formula: see text] where D-GlcN(L-Ala) is 2-(L-alanylamido)-2-deoxy-D-glucose.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Alanine / chemistry
Carbohydrate Sequence
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
O Antigens / chemistry*
Oligosaccharides / chemistry*
Proteus / chemistry*
Proteus / classification
Spectrometry, Mass, Fast Atom Bombardment

Substances

O Antigens
Oligosaccharides
Alanine