Activation of CPP32 and Mch3 alpha in wild-type p53-induced apoptosis

Biochem J. 1997 Feb 15;322 ( Pt 1)(Pt 1):19-23. doi: 10.1042/bj3220019.

Abstract

DNA-damaging agents induce apoptosis primarily by a p53-dependent pathway. LTR6 cells containing a temperature-sensitive p53 were used to dissect further the mechanisms of p53-induced apoptosis. Apoptosis was accompanied by the processing and activation of CPP32 and Mch3 alpha, together with the cleavage of poly(ADP-ribose) polymerase and lamin B1. These results demonstrate a critical role for the activation of interleukin-1 beta-converting enzyme-like proteases in p53-induced apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis* / drug effects
  • Caspase 3
  • Caspases*
  • Coumarins / metabolism
  • Cysteine Endopeptidases / metabolism*
  • Enzyme Activation / drug effects
  • Fluorescent Dyes
  • Leukemia, Myeloid
  • Mice
  • Oligopeptides / metabolism
  • Substrate Specificity
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / pharmacology*

Substances

  • Coumarins
  • Fluorescent Dyes
  • Oligopeptides
  • Tumor Suppressor Protein p53
  • acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin
  • Casp3 protein, mouse
  • Caspase 3
  • Caspases
  • Cysteine Endopeptidases