Molecular cloning and nucleotide sequencing of bioF (7-keto-8-amino pelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) genes of Erwinia herbicola

C H Wu; Y Y Bao; C P Shao; D Shiuan

doi:10.1080/15216549700201321

Molecular cloning and nucleotide sequencing of bioF (7-keto-8-amino pelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) genes of Erwinia herbicola

Biochem Mol Biol Int. 1997 Feb;41(2):311-5. doi: 10.1080/15216549700201321.

Authors

C H Wu¹, Y Y Bao, C P Shao, D Shiuan

Affiliation

¹ Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.

PMID: 9063571
DOI: 10.1080/15216549700201321

Abstract

The biotin operon of Erwinia herbicola Eho 10 was cloned and characterized by complementation of E. coli biotin mutants. The operon was found to contain five genes arranged in the order, bioABFCD. The nucleotide sequences of bioF (7-keto-8-aminopelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) were determined and analyzed. The nucleotide sequences and deduced amino acid sequences of bioFCD were compared with the corresponding sequences from Escherichia coli, Bacillus sphaericus, Serratia marcescens and Brevibacterium flavum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyltransferases / genetics*
Amino Acid Sequence
Base Sequence
Biotin / genetics*
Carbon-Nitrogen Ligases*
Erwinia / enzymology*
Erwinia / genetics*
Ligases / genetics*
Molecular Sequence Data
Operon
Sequence Homology

Substances

Biotin
Acyltransferases
8-amino-7-oxononanoate synthase
Ligases
Carbon-Nitrogen Ligases
dethiobiotin synthetase

Associated data

GENBANK/U51207
GENBANK/U51208