Abstract
We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions comparably to Bcl-2 and adenovirus E1B 19K in blocking apoptosis. However, GAM-1 has no sequence homology to Bcl-2, E1B 19K, or any other known antiapoptotic proteins and thus defines a novel antiapoptotic function.
MeSH terms
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Adenovirus E1B Proteins / physiology
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Amino Acid Sequence
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Apoptosis*
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Aviadenovirus / genetics*
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Cell Nucleus / virology
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DNA Nucleotidylexotransferase / metabolism
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Genes, Viral
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HeLa Cells
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Humans
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Molecular Sequence Data
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Mutagenesis
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Proto-Oncogene Proteins c-bcl-2 / physiology
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Sequence Homology, Amino Acid
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Signal Transduction
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Transfection
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Tumor Necrosis Factor-alpha / metabolism
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Viral Proteins / genetics*
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Viral Proteins / physiology
Substances
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Adenovirus E1B Proteins
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CELO protein, adenovirus
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Proto-Oncogene Proteins c-bcl-2
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Tumor Necrosis Factor-alpha
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Viral Proteins
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DNA Nucleotidylexotransferase