Clostridium perfringens toxins absorbed on filter paper--a simple method for sample conservation

Berl Munch Tierarztl Wochenschr. 1997 Feb;110(2):46-7.

Abstract

Clostridium perfringens type C and type D toxins were absorbed on filter paper, dried and stored at room temperature (18-20 degrees C), at 37 degrees C, at 4 degrees C and at -20 degrees C. Type specific toxin was correctly identified in the EIA for 74 days. Absorption on filter paper may offer a simple method for conservation and transport of post mortem samples from cases of suspected enterotoxaemia.

MeSH terms

  • Absorption
  • Animals
  • Animals, Domestic
  • Autopsy
  • Bacterial Toxins / analysis*
  • Cholesterol Esters / analysis*
  • Clostridium Infections / pathology
  • Clostridium Infections / veterinary
  • Clostridium perfringens*
  • Culture Media
  • Endotoxemia / pathology
  • Endotoxemia / veterinary
  • Immunoenzyme Techniques
  • Paper
  • Pyridinium Compounds / analysis*
  • Specimen Handling / methods

Substances

  • Bacterial Toxins
  • CPB protein, Clostridium perfringens
  • Cholesterol Esters
  • Culture Media
  • Pyridinium Compounds
  • cholesterylphosphorylethylpyridinium