Direct enantiomeric separation of cathinone and one major metabolite on cellobiohydrolase (CBH-I) chiral stationary phase

Biomed Chromatogr. 1997 Jan-Feb;11(1):47-9. doi: 10.1002/(SICI)1099-0801(199701)11:1<47::AID-BMC626>3.0.CO;2-M.

Abstract

A direct, isocratic high-performance liquid chromatographic (HPLC) method is described for the enantiomeric separation of racemic cathinone and one of its metabolites (+/-)-norephedrine, on a protein-based chiral stationary phase (CSP), cellobiohydrolase, known as CBH-I, under reversed-phase conditions. It was determined that the S-(-)-enantiomer eluted first for cathinone while the 1S,2R-(+)-enantiomer eluted first for norephedrine. The procedure is simple with no sample derivatization required and a short assay time.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / chemistry
  • Alkaloids / isolation & purification*
  • Cellulase*
  • Cellulose 1,4-beta-Cellobiosidase
  • Central Nervous System Stimulants / isolation & purification*
  • Chromatography, High Pressure Liquid / methods*
  • Osmolar Concentration
  • Phenylpropanolamine / chemistry
  • Phenylpropanolamine / isolation & purification*
  • Stereoisomerism

Substances

  • Alkaloids
  • Central Nervous System Stimulants
  • Phenylpropanolamine
  • cathinone
  • Cellulase
  • Cellulose 1,4-beta-Cellobiosidase