Abstract
A direct, isocratic high-performance liquid chromatographic (HPLC) method is described for the enantiomeric separation of racemic cathinone and one of its metabolites (+/-)-norephedrine, on a protein-based chiral stationary phase (CSP), cellobiohydrolase, known as CBH-I, under reversed-phase conditions. It was determined that the S-(-)-enantiomer eluted first for cathinone while the 1S,2R-(+)-enantiomer eluted first for norephedrine. The procedure is simple with no sample derivatization required and a short assay time.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / chemistry
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Alkaloids / isolation & purification*
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Cellulase*
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Cellulose 1,4-beta-Cellobiosidase
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Central Nervous System Stimulants / isolation & purification*
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Chromatography, High Pressure Liquid / methods*
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Osmolar Concentration
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Phenylpropanolamine / chemistry
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Phenylpropanolamine / isolation & purification*
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Stereoisomerism
Substances
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Alkaloids
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Central Nervous System Stimulants
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Phenylpropanolamine
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cathinone
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Cellulase
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Cellulose 1,4-beta-Cellobiosidase