Electrospray mass spectrometric characterization of a corticosteroid dimer

Rapid Commun Mass Spectrom. 1997;11(2):219-23. doi: 10.1002/(SICI)1097-0231(19970131)11:2<219::AID-RCM740>3.0.CO;2-O.

Abstract

18-Hydroxy-11-deoxycorticosterone forms a non-polar dimer under acidic conditions. The dimer was purified by high performance liquid chromatography and further analysed by electrospray mass spectrometry. The purified dimer has a molecular weight of 656 Da with two free ketone groups and no free hydroxyl functions. The mass spectrometric data suggest a 20,21,20',21'-anhydro dimer structure for the dimer. Microbore high performance liquid chromatography/electrospray mass spectrometry of rat adrenal extracts indicated that both 18-OH-B and 18-OH-DOC were present, although no 18-OH-DOC dimer was detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Hormones / chemistry*
  • Adrenal Cortex Hormones / isolation & purification
  • Adrenal Glands / chemistry
  • Animals
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • Mass Spectrometry
  • Rats
  • Rats, Wistar

Substances

  • Adrenal Cortex Hormones