Abstract
Genomic imprinting is an epigenetic modification in the germline leading to parental allele-specific gene expression in somatic cells. We have previously found that imprinted genes can be abnormally expressed or silenced in tumors and that the cyclin-dependent kinase inhibitor (CKI) CDKN1C (p57KIP2) is normally imprinted, with preferential expression of the maternal allele. Here we analyze the imprinting status of three additional CKIs, the abnormal expression and/or chromosomal localization of which has been implicated in human malignancy: CDKN1A, CDKN1B, and CDKN2C. Allele-specific expression was examined by reverse transcription-PCR, using primers that span transcribed polymorphisms as well as exon/intron boundaries, to distinguish cDNA products from genomic DNA. Biallelic expression was observed for all three genes in both fetal and adult tissues. Thus, genomic imprinting is not a generalized feature of CKIs.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Alleles
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Carrier Proteins / genetics*
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Cell Cycle Proteins*
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Cyclin-Dependent Kinase Inhibitor p18
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases / antagonists & inhibitors*
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Cyclins / genetics*
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Enzyme Inhibitors*
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Fungal Proteins / genetics*
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Gene Expression Regulation, Developmental
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Humans
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Imprinting, Psychological*
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Microtubule-Associated Proteins / genetics*
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Molecular Motor Proteins
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Polymorphism, Restriction Fragment Length
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RNA, Messenger / genetics
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Saccharomyces cerevisiae Proteins*
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Tumor Suppressor Proteins*
Substances
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CDKN1A protein, human
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CDKN2C protein, human
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Carrier Proteins
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p18
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Enzyme Inhibitors
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Fungal Proteins
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KIP2 protein, S cerevisiae
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Microtubule-Associated Proteins
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Molecular Motor Proteins
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Tumor Suppressor Proteins
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases