Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2 in Malayan spitting cobra (Naja naja sputatrix) venom

Toxicon. 1997 Jan;35(1):27-37. doi: 10.1016/s0041-0101(96)00071-2.

Abstract

cDNAs encoding three phospholipase A2 (PLA2) isoforms in Naja naja sputatrix were cloned and characterized. One of them encoded an acidic PLA2 (APLA) while the others encoded neutral PLA2 (NPLA-1 and NPLA-2). The specific characteristics of APLA and NPLA were attributed to mutations at nt139 and nt328 from G to C and G to A, respectively, resulting in amino acid substitutions from Asp20 and 83 in APLA to His20 and Asn83 in NPLA. Amino acid sequencing of purified protein also showed the presence of this Asp20 and His20 in APLA and NPLA, respectively. The cDNA encoding one of the PLA2 (NAJPLA-2A), when expressed in Escherichia coli, yielded a protein that exhibited PLA2 activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification*
  • Elapid Venoms / chemistry
  • Elapid Venoms / enzymology*
  • Elapid Venoms / genetics
  • Elapidae
  • Gene Expression Regulation
  • Genes
  • Isoenzymes / chemistry*
  • Isoenzymes / genetics*
  • Isoenzymes / isolation & purification
  • Molecular Sequence Data
  • Phospholipases A / chemistry*
  • Phospholipases A / genetics*
  • Phospholipases A / isolation & purification
  • Phospholipases A2
  • Recombinant Proteins / metabolism

Substances

  • DNA, Complementary
  • Elapid Venoms
  • Isoenzymes
  • Recombinant Proteins
  • Phospholipases A
  • Phospholipases A2

Associated data

  • GENBANK/L42004
  • GENBANK/L42005
  • GENBANK/L42006
  • GENBANK/X73225
  • GENBANK/X77755