An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria

J A Mangan; K M Sole; D A Mitchison; P D Butcher

doi:10.1093/nar/25.3.675

An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria

Nucleic Acids Res. 1997 Feb 1;25(3):675-6. doi: 10.1093/nar/25.3.675.

Authors

J A Mangan¹, K M Sole, D A Mitchison, P D Butcher

Affiliation

¹ Department of Medical Microbiology, St George's Hospital Medical School, Cranmer Terrace, London, SW17 0RE, UK.

Abstract

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Detergents / pharmacology
Escherichia coli Proteins*
HSP70 Heat-Shock Proteins / genetics
Molecular Sequence Data
Mycobacterium bovis / drug effects
Mycobacterium bovis / genetics*
RNA, Bacterial / isolation & purification*
RNA, Messenger / analysis
Streptococcus pneumoniae / genetics

Substances

Detergents
Escherichia coli Proteins
HSP70 Heat-Shock Proteins
RNA, Bacterial
RNA, Messenger
dnaK protein, E coli

Associated data

GENBANK/X58406