The proportion of bacteria exhibiting surface Fc gamma-binding proteins was determined in periodontal pockets of 20 patients diagnosed with periodontal disease and in subgingival areas of 20 patients without periodontal lesions. Bacterial smears were examined by fluorescence microscopy based on DNA staining (Hoechst 33256) and staining of Fc gamma-binding proteins by human biotin-labelled Fc gamma and Texas red-conjugated streptavidin. Fc gamma-binding proteins were observed in all smears from the patients diagnosed with periodontitis, and in a majority of the smears high proportions of the bacteria were positive for Fc gamma-binding proteins. In contrast, most smears from patients without periodontal lesions included low or undetectable proportions of bacteria with Fc gamma-binding proteins.