Purification and characterization of a lectin from Amaranthus hypochondriacus var. Mexico seeds

M Ozeki; K Kamemura; K Moriyama; Y Itoh; Y Furuichi; H Umekawa; T Takahashi

doi:10.1271/bbb.60.2048

Purification and characterization of a lectin from Amaranthus hypochondriacus var. Mexico seeds

Biosci Biotechnol Biochem. 1996 Dec;60(12):2048-51. doi: 10.1271/bbb.60.2048.

Authors

M Ozeki¹, K Kamemura, K Moriyama, Y Itoh, Y Furuichi, H Umekawa, T Takahashi

Affiliation

¹ Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.

PMID: 8988637
DOI: 10.1271/bbb.60.2048

Abstract

A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.

MeSH terms

Amino Acids / analysis
Animals
Chemical Phenomena
Chemistry, Physical
Electrophoresis, Polyacrylamide Gel
Erythrocytes / drug effects
Haptens / analysis
In Vitro Techniques
Isoelectric Focusing
Lectins / analysis*
Lectins / isolation & purification
Lectins / pharmacology
Molecular Weight
Plant Lectins
Plants / chemistry*
Rabbits
Seeds / chemistry*

Substances

Amino Acids
Haptens
Lectins
Plant Lectins