A novel host factor for integration of mycobacteriophage L5

Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15411-6. doi: 10.1073/pnas.93.26.15411.

Abstract

Bacterial integration host factors (IHFs) play central roles in the cellular processes of recombination, DNA replication, transcription, and bacterial pathogenesis. We describe here a novel mycobacterial IHF (mIHF) of Mycobacterium smegmatis and Mycobacterium tuberculosis that stimulates integration of mycobacteriophage L5. mIHF is the product of a single gene and is unrelated at the sequence level to other integration host factors. By itself, mIHF does not bind preferentially to attP DNA, although it significantly alters the pattern of integrase (Int) binding, promoting the formation of specific integrase-mIHF-attP intasome complexes.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / isolation & purification
  • Base Sequence
  • DNA Footprinting
  • DNA-Binding Proteins / biosynthesis
  • Deoxyribonuclease I
  • Genes, Viral
  • Integration Host Factors
  • Molecular Sequence Data
  • Mycobacteriophages / genetics
  • Mycobacteriophages / physiology
  • Mycobacterium / virology*
  • Mycobacterium tuberculosis / virology*
  • Recombination, Genetic
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Integration Host Factors
  • Deoxyribonuclease I

Associated data

  • GENBANK/U75344
  • GENBANK/Z80108