Abstract
Using the Semliki Forest virus (SFV) and Sindbis virus (SIN) cDNAs we have constructed recombinants in which the spike genes were exchanged. Analyses of expression showed that the SFV/SIN(spike) RNA directed efficient assembly of infectious virus, whereas the reciprocal SIN/SFV(spike) RNA was completely unable to assemble virus. This was apparently due to a defective capsid-spike interaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line
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Cricetinae
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Molecular Sequence Data
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Reassortant Viruses / genetics
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Reassortant Viruses / physiology*
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Recombination, Genetic
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Semliki forest virus / genetics
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Semliki forest virus / physiology*
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Sindbis Virus / genetics
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Sindbis Virus / physiology*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / metabolism*
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Viral Structural Proteins / biosynthesis
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Virus Assembly*
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Virus Replication
Substances
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Viral Envelope Proteins
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Viral Structural Proteins