Fusion between Jurkat cell and PEO-lipid modified liposome

Biochim Biophys Acta. 1996 Dec 4;1285(2):183-91. doi: 10.1016/s0005-2736(96)00159-9.

Abstract

Direct fusion between Jurkat cell and a liposome modified with poly(ethylene oxide)-bearing lipid (PEO-lipid) was examined using diphtheria toxin fragment A (DTA) as the probe. Only the DTA-loaded liposome modified with PEO-lipid(n = 32) (n is the number of ethylene oxide units) exerted significant cytotoxicity against Jurkat cells, while liposomes lacking either the PEO-lipid or DTA did not. Liposomes modified by the PEO-lipid with shorter PEO chain(n = 5 or 15) did not show any cytotoxicity, irrespective of their DTA-loading. The cytotoxicity was observed even in the presence of cytochalasin B, an inhibitor of endocytosis. Judging from these results, we concluded that the PEO-lipid(n = 32)-modified liposome directly fused with plasma membrane of Jurkat cell.

MeSH terms

  • Cell Survival / drug effects
  • Culture Media / pharmacology
  • Cytochalasin B / pharmacology
  • Diphtheria Toxin / metabolism
  • Diphtheria Toxin / toxicity*
  • Endocytosis / drug effects
  • Humans
  • Liposomes / metabolism*
  • Membrane Fusion / physiology*
  • Microscopy, Phase-Contrast
  • Molecular Structure
  • Peptide Fragments / metabolism
  • Peptide Fragments / toxicity*
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / pharmacology*
  • Triglycerides / chemistry
  • Triglycerides / pharmacology
  • Tumor Cells, Cultured

Substances

  • Culture Media
  • Diphtheria Toxin
  • Liposomes
  • Peptide Fragments
  • Triglycerides
  • diphtheria toxin fragment A
  • Cytochalasin B
  • Polyethylene Glycols