Abstract
A kinetic analysis of secretion of lipo-chitin oligosaccharides (LCO) produced by Rhizobium etli (Re) wild-type (wt) strain and derivatives carrying disrupted nodI or nodJ genes was performed. LCO were detected in the growth media of the wt strain as early as 1 h after nod gene induction. In contrast, strains carrying nodI or nodJ mutations secreted less LCO, and accumulated LCO metabolites intracellularly after 4 h of induction. These Re mutants presented a delayed nodulation phenotype and a reduction in the maximum number of nodules formed in Phaseolus vulgaris roots.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters*
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Antigens, Bacterial / metabolism*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Biological Transport
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Chitin / metabolism*
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Chromatography, Thin Layer
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Fabaceae / microbiology
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Genes, Bacterial
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Kinetics
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Lipopolysaccharides / metabolism*
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Membrane Transport Proteins
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Mutation
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Nitrogen Fixation
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Plants, Medicinal
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Rhizobium / genetics
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Rhizobium / metabolism*
Substances
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ATP-Binding Cassette Transporters
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Antigens, Bacterial
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Bacterial Proteins
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Carrier Proteins
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Lipopolysaccharides
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Membrane Transport Proteins
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lipid-linked oligosaccharides
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nodI protein, Bacteria
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nodJ protein, Rhizobium etli
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Chitin