The role of the nodI and nodJ genes in the transport of Nod metabolites in Rhizobium etli

Gene. 1996 Sep 16;173(2):183-7. doi: 10.1016/0378-1119(96)00166-7.

Abstract

A kinetic analysis of secretion of lipo-chitin oligosaccharides (LCO) produced by Rhizobium etli (Re) wild-type (wt) strain and derivatives carrying disrupted nodI or nodJ genes was performed. LCO were detected in the growth media of the wt strain as early as 1 h after nod gene induction. In contrast, strains carrying nodI or nodJ mutations secreted less LCO, and accumulated LCO metabolites intracellularly after 4 h of induction. These Re mutants presented a delayed nodulation phenotype and a reduction in the maximum number of nodules formed in Phaseolus vulgaris roots.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Antigens, Bacterial / metabolism*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biological Transport
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Chitin / metabolism*
  • Chromatography, Thin Layer
  • Fabaceae / microbiology
  • Genes, Bacterial
  • Kinetics
  • Lipopolysaccharides / metabolism*
  • Membrane Transport Proteins
  • Mutation
  • Nitrogen Fixation
  • Plants, Medicinal
  • Rhizobium / genetics
  • Rhizobium / metabolism*

Substances

  • ATP-Binding Cassette Transporters
  • Antigens, Bacterial
  • Bacterial Proteins
  • Carrier Proteins
  • Lipopolysaccharides
  • Membrane Transport Proteins
  • lipid-linked oligosaccharides
  • nodI protein, Bacteria
  • nodJ protein, Rhizobium etli
  • Chitin