In this research we have investigated the effects of mitochondrial peptide fraction bound to DNA on stationary rat hepatocyte cultures. The treatment with this peptide was responsible of cellular lysis, followed by significant proliferation after 46th day until 51st in which it is reestablished. In the present study it seems that peptides bound to hepatocyte mitochondrial DNA are involved in longer term cultural stabilization properties. Our results demonstrate that the peptide is a lysing factor activity at short terms.