Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes

Yeast. 1996 Nov;12(14):1475-81. doi: 10.1002/(sici)1097-0061(199611)12:14<1475::aid-yea32>3.0.co;2-v.

Abstract

We have determined the nucleotide sequence of a 40 kb fragment from the right arm of chromosome XV of Saccharomyces cerevisiae. Subsequent analysis revealed 18 non-overlapping open reading frames (ORFs) numbered from 06257 to 06357, an ARS, two tRNA genes and a Ty2 with its flanking elements. Ten ORFs have been sequenced previously: TEA1, RPA43, RPA190, SGC1 (also called TYE7) REV1, PUT4, CIN1, MNE and MRE4 (also called MEK1). Among the others, two seem to code for a new pyruvate kinase and for a new ubiquitin-conjugating enzyme; three have interesting homology with genes located on the left arm of chromosome I. This similarity with chromosome I extends to the left of the sequence presented here (Parle et al., submitted to Yeast). The homologous genes on the two chromosomes are placed in the same relative order.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Fungal / genetics*
  • DNA, Fungal / genetics*
  • Genes, Fungal
  • Molecular Sequence Data
  • Open Reading Frames
  • Pyruvate Kinase / genetics
  • RNA, Transfer / genetics
  • Saccharomyces cerevisiae / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • RNA, Transfer
  • Pyruvate Kinase

Associated data

  • GENBANK/X95720