Abstract
In nematodes, flies, and mammals, dosage compensation equalizes X-chromosome gene expression between the sexes through chromosome-wide regulatory mechanisms that function in one sex to adjust the levels of X-linked transcripts. Here, a dosage compensation complex was identified in the nematode Caenorhabditis elegans that reduces transcript levels from the two X chromosomes in hermaphrodites. This complex contains at least four proteins, including products of the dosage compensation genes dpy-26 and dpy-27. Specific localization of the complex to the hermaphrodite X chromosomes is conferred by XX-specific regulatory genes that coordinately control both sex determination and dosage compensation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins*
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Carrier Proteins / analysis
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Carrier Proteins / chemistry
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Carrier Proteins / metabolism*
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Disorders of Sex Development
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Dosage Compensation, Genetic*
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Electrophoresis, Polyacrylamide Gel
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Female
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Genes, Helminth
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Genes, Regulator
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Helminth Proteins / analysis
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Helminth Proteins / chemistry
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Helminth Proteins / metabolism*
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Male
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Nuclear Proteins / analysis
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Nuclear Proteins / chemistry
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Nuclear Proteins / metabolism*
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Precipitin Tests
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RNA, Helminth / metabolism
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RNA, Messenger / metabolism
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Sex Determination Analysis
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X Chromosome / chemistry
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X Chromosome / metabolism*
Substances
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Caenorhabditis elegans Proteins
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Carrier Proteins
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DPY-27 protein, C elegans
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Helminth Proteins
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Nuclear Proteins
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RNA, Helminth
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RNA, Messenger