Abstract
beta-Glucosidase was extracted from sonicated Bifidobacterium adolescentis Int-57 and partially purified by Sepharose CL-6B gel-filtration and DEAE-cellulose ion-exchange chromatography. The partially purified enzyme was confirmed to convert cycasin to a mutagen in the Ames and SOS chromotests. beta-Glucosidase negative strains were unable to activate cycasin mutagenically.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bifidobacterium / enzymology*
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Biodegradation, Environmental
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Cycasin / metabolism*
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Feces / chemistry
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Fermentation
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Food Microbiology
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Humans
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In Vitro Techniques
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Mutagenicity Tests
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Mutagens / metabolism*
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Substrate Specificity
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beta-Glucosidase / isolation & purification
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beta-Glucosidase / metabolism*
Substances
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Mutagens
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Cycasin
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beta-Glucosidase