A partially purified beta-glucosidase from Bifidobacterium adolescentis converts cycasin to a mutagenic compound

Lett Appl Microbiol. 1996 Feb;22(2):145-8. doi: 10.1111/j.1472-765x.1996.tb01129.x.

Abstract

beta-Glucosidase was extracted from sonicated Bifidobacterium adolescentis Int-57 and partially purified by Sepharose CL-6B gel-filtration and DEAE-cellulose ion-exchange chromatography. The partially purified enzyme was confirmed to convert cycasin to a mutagen in the Ames and SOS chromotests. beta-Glucosidase negative strains were unable to activate cycasin mutagenically.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bifidobacterium / enzymology*
  • Biodegradation, Environmental
  • Cycasin / metabolism*
  • Feces / chemistry
  • Fermentation
  • Food Microbiology
  • Humans
  • In Vitro Techniques
  • Mutagenicity Tests
  • Mutagens / metabolism*
  • Substrate Specificity
  • beta-Glucosidase / isolation & purification
  • beta-Glucosidase / metabolism*

Substances

  • Mutagens
  • Cycasin
  • beta-Glucosidase