This paper presents the nucleotide sequences of the cDNAs encoding three acidic phospholipases A2 and one basic phospholipase A2 from Deinagkistrodon acutus venom. The deduced primary structure of the basic enzyme is closest to that of the basic neurotoxic enzyme from Trimeresurus mucrosquamatus venom, while the acidic phospholipases from D. acutus have highest sequence similarity to that from Agkistrodon halys pallas. The phylogeny of this monotypic species is discussed.