A virus isolated from vaginal exudate of cows with vaginitis showed molecular weight around 92 to 143 x 10(3) KD, density between 1.168 and 1.203 g/ml and physical, chemical and morphological properties characteristic of virus belonging to the herpes group. This virus was not reagent with the fluorescent antibodies of Infectious Bovine Rhinotracheitis (IBR) or Parainfluenza-3 (PI-3) viruses, frequently detected in cows with vaginitis.