Abstract
Macrolide 2'-phosphotransferase II [MPH(2')II] inactivates macrolide antibiotics. The mphB gene for MPH(2')II was cloned from Escherichia coli and sequenced. Analysis of the nucleotide sequence indicated that mphB encoded a protein of 302 amino acids with a molecular mass of 34483 Da. The carboxy terminal region of the deduced protein contained a sequence that resembled a conserved functional domain in aminoglycoside phosphotransferases.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins
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Genes, Bacterial*
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Molecular Sequence Data
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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Phosphotransferases (Alcohol Group Acceptor)
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macrolide 2'-kinase
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mphB protein, E coli