Abstract
The Aspergillus nidulans stcP gene was previously identified as a transcribed region associated with a cluster of genes proposed to be involved in sterigmatocystin biosynthesis (D. W. Brown, J.-H. Yu, H. S. Kelkar, M. Fernandes, T. C. Nesbitt, N. P. Keller, T. H. Adams, and T. J. Leonard, Proc. Natl. Acad. Sci. USA 93:1418-1422, 1996). stcP was predicted to encode a methyltransferase responsible for conversion of demethylsterig-matocystin to sterigmatocystin. Here we demonstrate that disruption of stcP in A. nidulans results in strains that accumulate demethylsterigmatocystin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Aspergillus nidulans / enzymology*
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Aspergillus nidulans / genetics*
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Aspergillus nidulans / metabolism
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Escherichia coli / genetics
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Fungal Proteins*
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Genes, Fungal*
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Methyltransferases / genetics*
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Methyltransferases / metabolism*
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Molecular Sequence Data
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Multigene Family
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Mutagenesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
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Sterigmatocystin / biosynthesis*
Substances
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Fungal Proteins
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Recombinant Fusion Proteins
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Sterigmatocystin
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Methyltransferases
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omtA protein, Aspergillus