Of 220 wild Eastern Barred Bandicoots (Perameles gunnii) captured in two locations in Tasmania, Australia, 55 (25%) had a detectable parasitaemia with gametocytes of the protozoan genus Hepatozoon in blood films. Blood films were stained with Leishman stain and acridine orange O. Gamonts were commonly found as intracellular forms and occasionally observed as extracellular forms in peripheral erythrocytes. This is the first record of a member of the genus Hepatozoon in any temperate bandicoot and additionally appears to be the first record of this protozoan genus in any native marsupial species of Tasmania.