Salmonella enteritidis, phage type 4 (SE PT4), was isolated from five of six 27-wk-old layer chickens submitted for necropsy from a flock of 43,000. Bacteriologic and epidemiologic investigations on the ranch revealed that five of the eight flocks (n = 176,000) were infected. The prevalence of SE PT4 in randomly selected healthy birds ranged from 1.7% (in caged birds) to 50% (in free-range birds) and prevalence in culled birds (kept on dirt floor houses) ranged from 14% to 42%. The estimated overall prevalence of group D Salmonella in eggs contaminated with group D Salmonella was 2.28 per 10,000. The estimated prevalence of group D Salmonella in eggs from caged birds in three infected houses ranged from 1.5 to 4.1 per 10,000, whereas in two houses of free-range birds, prevalence was 14.9 to 19.1 per 10,000. Three of the eight flocks on the ranch remained negative for Salmonella between May 1994 and December 1995 or until removed from the ranch. Salmonella enteritidis PT4 was also isolated from 12.5% (6 of 48) of mice; 57% (four of seven) of cats; and two of two skunks tested. Environmental drag swabs and well water samples yielded multiple serotypes of Salmonella (23/180 and 5/14, respectively) but not S. enteritidis.