The pupation site preference and the quantity of larval salivary gland secretion proteins (glue proteins) were analysed in 15 species of Drosophila belonging to 3 species groups. The results showed that even under constant environmental conditions, the larvae of different species prefer to pupate at different sites in various proportions. Three patterns of pupation site preference could be recognized on the basis of the preponderance of larvae pupating at different sites. The classification of different species of Drosophila under study into 3 pattern groups does not correspond with their taxonomic classification. The larvae of those species that synthesize large quantities of glue protein tend to pupate in the medium, while those synthesizing half the quantity of glue proteins pupate on the sides of the container (glass wall). The quantity of glue protein is not correlated with the size of the salivary glands.