We investigated the chromosomal loss pattern of a primary large nodule and one of the disseminated intrahepatic small nodules of hepatocellular carcinoma in one patient using RFLP analysis. Using D17S30 probe mapping to 17p, loss of heterozygosity was observed both the main nodule and the disseminated nodule, however, using IGLV probe mapping to 22q, loss of heterozygosity was observed only in the disseminated nodule. This is the case in which serial loss of chromosome in the progression on hepatocellular carcinoma was highly suggested.