Abstract
Tat may stimulate transcriptional elongation by recruitment of a complex containing Tat-SF1 and a kinase to the human immunodeficiency virus-type 1 (HIV-1) promoter through a Tat-TAR interaction. A complementary DNA for the cellular activity, Tat-SF1, has been isolated. This factor is required for Tat trans-activation and is a substrate of an associated cellular kinase. Cotransfection with the complementary DNA for Tat-SF1 specifically modulates Tat activation. Tat-SF1 contains two RNA recognition motifs and a highly acidic carboxyl-terminal half. It is distantly related to EWS and FUS/TLS, members of a family of putative transcription factors with RNA recognition motifs that are associated with sarcomas.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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DNA, Complementary / genetics
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Electrophoresis, Polyacrylamide Gel
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Gene Expression
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Gene Products, tat / genetics*
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HIV Long Terminal Repeat
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HIV-1 / genetics*
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HeLa Cells
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Heterogeneous-Nuclear Ribonucleoproteins
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Humans
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Immunoblotting
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Molecular Sequence Data
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Molecular Weight
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Neoplasm Proteins / chemistry
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Phosphorylation
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Promoter Regions, Genetic
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Protein Kinases / metabolism
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RNA, Viral / metabolism
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RNA-Binding Protein EWS
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RNA-Binding Protein FUS
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Ribonucleoproteins / chemistry
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Sequence Homology, Amino Acid
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Trans-Activators / chemistry
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Trans-Activators / genetics*
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Trans-Activators / metabolism
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Transcriptional Activation*
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Transfection
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tat Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Complementary
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Gene Products, tat
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HTATSF1 protein, human
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Heterogeneous-Nuclear Ribonucleoproteins
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Neoplasm Proteins
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RNA, Viral
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RNA-Binding Protein EWS
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RNA-Binding Protein FUS
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Ribonucleoproteins
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Trans-Activators
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tat Gene Products, Human Immunodeficiency Virus
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Protein Kinases