Abstract
Uptake of [U-14C] glycine during the organophosphorus-ester-induced delayed neurotoxicity (OPIDN) development period was studied. Diisopropyl fluorophosphate (DFP), a delayed neurotoxic organophosphorus ester was administered to adult rats and hens. Results showed a decreased accumulation of glycine in hen cerebral cortex slices during the delayed neurotoxicity development period. An altered sensitivity toward transport inhibitors 2,4-dinitrophenol and ouabain was observed in DFP-treated hens. An altered neuronal membrane function during the OPIDN development period is reported in the present work. Brain Na+, K(+)-ATPase and Ca(++)-ATPase activities decreased during the neurotoxicity development period. The decrease in Ca(++)-ATPase activity persisted in hens until the complete development of neurotoxic symptoms. Decreased Ca++ pump activity is correlated with altered membrane function during OPIDN.
MeSH terms
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2,4-Dinitrophenol
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Animals
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Brain Chemistry / drug effects*
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Calcium-Transporting ATPases / antagonists & inhibitors
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Calcium-Transporting ATPases / drug effects*
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Calcium-Transporting ATPases / metabolism
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism
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Chickens
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Dinitrophenols / pharmacology
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Enzyme Inhibitors / pharmacology
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Female
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Glycine / metabolism*
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In Vitro Techniques
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Isoflurophate / pharmacology
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Nervous System Diseases / chemically induced*
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Nervous System Diseases / enzymology
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Nervous System Diseases / pathology
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Organophosphorus Compounds / pharmacokinetics
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Organophosphorus Compounds / pharmacology*
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Ouabain / pharmacology
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Protease Inhibitors / pharmacology
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Rats
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Rats, Wistar
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Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
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Sodium-Potassium-Exchanging ATPase / drug effects*
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Sodium-Potassium-Exchanging ATPase / metabolism
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Uncoupling Agents / pharmacology
Substances
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Dinitrophenols
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Enzyme Inhibitors
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Organophosphorus Compounds
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Protease Inhibitors
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Uncoupling Agents
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Isoflurophate
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Ouabain
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Calcium-Transporting ATPases
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Sodium-Potassium-Exchanging ATPase
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2,4-Dinitrophenol
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Glycine