Abstract
Following the identification of a M. tuberculosis phosphate transporter belonging to the superfamily of ABC transporters, we report on the cloning and sequencing of two additional genes, called pstS-3 and pstC-2, encoding proteins homologous to PstS and PstC of Escherichia coli, respectively. Together with the previously isolated M. tuberculosis gene similar to the E. coli pstA, these are included in a cluster encoding a second putative phosphate transport system. We demonstrate that pstS-3 encodes the previously described Ag 88, a 40 kDa M. bovis BCG culture filtrate antigen (immunodominant in H-2b haplotype type mice). Finally, a signature motif identifying integral transmembrane proteins of prokaryotic phosphate binding-dependent permeases is proposed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters / chemistry
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ATP-Binding Cassette Transporters / genetics*
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Binding Sites
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Cloning, Molecular
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Conserved Sequence
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Cyanogen Bromide
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Electrophoresis, Polyacrylamide Gel
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Genes, Bacterial
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Immunoblotting
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Molecular Sequence Data
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Multigene Family*
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Mycobacterium tuberculosis / chemistry
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Mycobacterium tuberculosis / genetics*
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Operon
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Peptides / chemistry
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Phosphate-Binding Proteins
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Sequence Analysis, DNA
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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Carrier Proteins
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Peptides
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Phosphate-Binding Proteins
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PstC-2 protein, Mycobacterium tuberculosis
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PstS-3 protein, Mycobacterium tuberculosis
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Cyanogen Bromide
Associated data
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GENBANK/X75297
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GENBANK/Z47983
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GENBANK/Z48057